Amelia Whitehart August 15, 2021

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Accordingly, the differential gene expressions of those transcription factor genes are brought about by the changes in ecdysteroid titer. By contrast, when ASGs were transferred differentially to a hormone-free medium, 20E was revealed to be required until 42 h of the culture for competing PCD. These results suggest that PCD of ASGs is governed by two different molecular mechanisms, one associated with de novo gene expression and the other mediated by nongenomic action of 20E.

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We are interested to develop the method working the gene at late stage of development. The inducible RNAi using the transgenic organism was known to be useful to analyze the function of the late genes. We first constructed a new vector to transcribe the RNA with hairpin structure of the target genes in the transgenic silkworm. The vector contains a marker gene of 3XP3GFP and the silkworm cytoplasmic actin intron to transport the transcribed RNA to cytoplasm. The introduced gene is placed under the control of UAS in opposite direction and driven by GAL4.